Journal: Antibiotics

Article Title: Antimicrobial Resistance Surveillance of Pigs and Chickens in the Lao People’s Democratic Republic, 2018–2021

doi: 10.3390/antibiotics11020177

Figure Lengend Snippet: Distribution of minimum inhibitory concentration (MIC) in a panel of 14 antimicrobials, showing the relative locations of the clinical breakpoints and the median MIC values in Escherichia coli isolated from chickens ( n = 1110 isolates) and pigs ( n = 754).

Article Snippet: The susceptibility of E. coli and Salmonella spp. isolates was determined using the commercial Sensititre TM Minimum Inhibitory Concentration (MIC) EUVSEC plates (Thermo Fisher Scientific, Waltham, MA, USA) [ ].

Techniques: Concentration Assay, Isolation

Journal: Antibiotics

Article Title: Antimicrobial Resistance Surveillance of Pigs and Chickens in the Lao People’s Democratic Republic, 2018–2021

doi: 10.3390/antibiotics11020177

Figure Lengend Snippet: Distribution of minimum inhibitory concentrations (MICs) in a panel of 14 antimicrobials, showing the relative locations of the clinical breakpoints and the median MIC values in Salmonella spp. isolated from chickens ( n = 698 isolates) and pigs ( n = 673).

Article Snippet: The susceptibility of E. coli and Salmonella spp. isolates was determined using the commercial Sensititre TM Minimum Inhibitory Concentration (MIC) EUVSEC plates (Thermo Fisher Scientific, Waltham, MA, USA) [ ].

Techniques: Isolation

Journal: Nature Communications

Article Title: CAR-T cell therapy targeting surface expression of TYRP1 to treat cutaneous and rare melanoma subtypes

doi: 10.1038/s41467-024-45221-2

Figure Lengend Snippet: a Kinetic of tumor growth or regression over time after treatment with 20D7SL-28ζ CAR-T cells. Tumor size was measured with a caliper. Mean ± SEM is plotted ( n = 10 mice for untreated, n = 11 mice for UTD and 20D7SL-28z). **** p < 0.0005 vs Untreated, #### p < 0.0005 vs UTD, unpaired, two-tailed t test with Holm-Sidak adjustment for multiple comparisons. Retrovirus vector copy number in the tumor ( b ) and the spleen ( c ) of mice treated with 20D7SL-28ζ CAR-T cell therapy at days 5, 13, and 27 after adoptive T-cell transfer. Mean ± SD is plotted ( n = 10 mice). Complete blood counts at days 5, 13, and 27 after adoptive T-cell transfer with 20D7SL-28ζ CAR-T cells. White blood cells ( d ), hemoglobin ( e ), hematocrit ( f ), and Platelets ( g ) are shown. Mean ± SD are plotted, each dot is one mouse ( d – f) Untreated n = 9 for Day 6 and n = 10 for Day 13, UTD n = 8 for Day 6 and n = 10 for Day 13, 20D7SL-28z n = 8 for Day 6, n = 6 for Day 13 and n = 7 for Day 27; ( g ) Untreated n = 8 for Day 6 and n = 9 for Day 13, UTD n = 8 for Day 6 and n = 9 for Day 13, 20D7SL-28z n = 6 for Day 6, n = 2 for Day 13 and n = 6 for Day 27). Serum chemistry at day 13, after adoptive T-cell transfer with 20D7SL-28ζ CAR-T cells. Mice with tumors treated with untransduced T cells and vehicle were used as controls. Untreated mice without tumors and mice without tumors receiving irradiation only were used as additional controls. Alkaline phosphatase ( h ), Alanine aminotransferase ( i ), aspartate aminotransferase ( j ), and total bilirubin ( k ) are shown. Mean ± SD is plotted ( n = 5 mice). Cytokine release in serum at days 5, 13, and 27 after adoptive T-cell transfer with 20D7SL-28ζ CAR-T cells. IL-6 ( l ), IFNγ ( m ), and IL-10 ( n ) are shown. Mean ± SD are plotted, each dot is one mouse ( l – n ) Untreated n = 7 for Day 5 and n = 9 for Day 13, UTD n = 7 for Day 6 and n = 10 for Day 13, 20D7SL-28z n = 7 for Day 6, n = 6 for Day 13 and n = 10 for Day 27). Black dashed line shows the minimum quantifiable levels using the BD CBA Mouse Inflammation Kit (20 pg/mL). o , p Quantitative measures of the average ONL thickness were taken at 0.2 mm intervals in frames (−10 to 10) to the optic nerve. Mean ± SEM is plotted ( n = 4 mice). q Quantitative TYRP1 pixel intensity in the RPE cells from mice at day 13 and 27 after receiving ACT with untransduced T cells or 20D7SL-28ζ CAR-T cells. Mean ± SD is plotted ( n = 4 mice, three sections of the retina per mouse). * p < 0.05, ** p < 0.005, *** p < 0.001, **** p < 0.0005 unpaired, two-tailed t test with Holm-Sidak adjustment for multiple comparisons. Unless otherwise indicated, differences are not statistically significant. Unless otherwise indicated, untransduced T cells and PBS were used as controls. Source data and exact p values are provided as a file.

Article Snippet: Black dashed line shows the minimum quantifiable levels using the BD CBA Mouse Inflammation Kit (20 pg/mL). o , p Quantitative measures of the average ONL thickness were taken at 0.2 mm intervals in frames (−10 to 10) to the optic nerve.

Techniques: Two Tailed Test, Plasmid Preparation, Irradiation